Hepatitis B Virus Combo Test

Npaj siv

Hepatitis B Virus Combo Test (Tag Nrho Cov Ntshav / Ntshav / Ntshav) yog qhov ntsuas chromatographic immunoassay sai rau kev kuaj pom zoo ntawm HBsAg, HBsAb, HBeAg, HBeAb thiab HBcAb hauv cov ntshav, ntshav lossis ntshav. Tsis txhob siv Hepatitis B Virus Combo Test yog tias lub hnab ntawv nyiaj puas lawm.

Txoj Cai

HBsAg thiab HBeAg

Cov kev ntsuam xyuas HBsAg thiab HBeAg yog qhov zoo, ob-site sandwich immunoassays rau kev kuaj pom HBsAg lossis HBeAg hauv ntshav lossis ntshav. Lub daim nyias nyias yog pre-coated nrog anti-HBsAg los yog anti-HBeAg cov tshuaj tiv thaiv ntawm thaj tsam kab mob ntawm lub sawb. Thaum kuaj, cov kua dej los yog cov ntshav plasma reacts nrog cov particle coated nrog anti-HBsAg los yog anti-HBeAg cov tshuaj tiv thaiv. tsim kom muaj ib txoj kab xim. Lub xub ntiag ntawm cov kab xim no nyob rau hauv cheeb tsam kev xeem kab qhia tau hais tias muaj txiaj ntsig zoo, thaum nws qhov tsis tuaj yeem qhia tias qhov tshwm sim tsis zoo.

HBsAb

Hepatitis B nto Antibody (HBsAb) kuj hu ua anti-Hepatitis B nto Antigen (anti-HBs). Qhov kev sim no yog qhov zoo, lateral flow immunoassay rau kev kuaj pom HBsAb hauv cov ntshav los yog ntshav. Lub daim nyias nyias yog pre-coated nrog HBsAg ntawm cov kab xeem kab ntawm lub sawb. Thaum kuaj, cov ntshav los yog cov ntshav plasma reacts nrog cov particle coated nrog HBsAg. Qhov sib xyaw ua ke nce mus rau ntawm daim nyias nyias chromatographically los ntawm capillary ua kom hnov ​​​​mob nrog HBsAg ntawm daim nyias nyias thiab tsim cov kab xim. Lub xub ntiag ntawm cov kab xim no nyob rau hauv cheeb tsam kev xeem kab qhia tau hais tias muaj txiaj ntsig zoo, thaum nws qhov tsis tuaj yeem qhia tias qhov tshwm sim tsis zoo.

HBeAb and HBcAb

Hepatitis B hnab ntawv Antibody (HBeAb) tseem hu ua anti-Hepatitis B hnab ntawv Antigen (anti-HBe). Hepatitis B core Antibody (HBcAb) tseem hu ua anti-Hepatitis B core Antigen (anti-HBc). Cov kev sim no yog immunoassays raws li lub hauv paus ntsiab lus ntawm kev sib tw binding.

Thaum kuaj, qhov sib xyaw ua ke nce mus rau ntawm daim nyias nyias chromatographically los ntawm kev ua ntawm capillary. Lub membrane yog pre-coated nrog HBeAg los yog HBcAg nyob rau hauv lub xeem kab cheeb tsam ntawm lub sawb. Thaum kuaj, anti-HBe antibody los yog anti-HBc antibody, yog muaj nyob rau hauv cov qauv, yuav sib tw nrog particle coated anti-HBe antibody los yog anti-HBc antibody rau tsawg npaum li cas ntawm HBeAg los yog HBcAg ntawm daim nyias nyias, thiab tsis muaj kab yuav tsim nyob rau hauv thaj tsam kab xeem, qhia txog qhov txiaj ntsig zoo. Ib txoj kab uas pom muaj xim yuav tsim nyob rau hauv cheeb tsam xeem kab yog tsis muaj anti-HBe antibody los yog anti-HBc antibody nyob rau hauv cov qauv vim hais tias tag nrho cov antibody coated hais yuav raug ntes los ntawm cov antigen coated nyob rau hauv lub xeem kab.

Txhawm rau ua raws li cov txheej txheem tswj hwm, cov kab xim xim yuav ib txwm tshwm sim hauv cheeb tsam tswj kab uas qhia tau hais tias qhov ntim ntawm cov qauv raug ntxiv thiab daim nyias nyias wicking tau tshwm sim.

Cov txheej txheem xeem

Nqa cov kev ntsuam xyuas, cov qauv, thiab/lossis tswj rau hauv chav tsev kub (15-30 degree) ua ntej siv.

1. Tshem tawm qhov kev xeem los ntawm nws lub hnab ntim khoom, thiab muab tso rau ntawm qhov chaw huv, theem. Txhawm rau kom tau txais txiaj ntsig zoo tshaj plaws, qhov kev ntsuam xyuas yuav tsum tau ua hauv ib teev.

2. Muab cov cuab yeej xeem tso rau ntawm qhov chaw huv, theem. Sau npe nrog tus lej ID.

Rau cov ntshav venous tag nrho cov qauv:

Tuav lub dropper vertically thiab hloov 2 tee ntawm cov ntshav tag nrho (kwv yees li 50 µL) mus rau cov qauv zoo (S) ntawm cov cuab yeej kuaj, tom qab ntawd ntxiv 1 tee ntawm qhov tsis muaj (kwv yees li 40 µL) raws li, thiab pib lub sijhawm.

Rau cov ntiv tes tag nrho cov ntshav kuaj:

Dispense 2 tee ntawm tus ntiv tes tag nrho cov ntshav mus rau hauv cov qauv zoo (S) ntawm cov cuab yeej kuaj, tom qab ntawd ntxiv 1 poob ntawm qhov tsis sib xws (kwv yees li 40 µL) raws li, thiab pib lub sijhawm.

Rau cov ntshav plasma

Siv cov khoom siv pov tseg pov tseg, hloov 3 tee ntshav los yog ntshav ntshav (kwv yees li 75 µL) rau txhua tus qauv zoo (S) ntawm lub cuab yeej raws li, tom qab ntawd pib lub sijhawm.

Tsis txhob tso cua npuas hauv qhov hnoos qeev (S), thiab tsis txhob ntxiv cov tshuaj rau lub qhov rais tshwm sim.

Raws li qhov kev sim pib ua haujlwm, xim yuav txav mus rau hauv daim nyias nyias.

3. Tos kom cov xim dawb tshwm tuaj. Cov txiaj ntsig yuav tsum tau nyeem ntawm 15 feeb.

Cim npe nrov: kab mob siab b ib tus kab mob sib tes, Suav teb, neeg muab khoom, manufacturers, Hoobkas, loj