SARS-CoV-2 Nucleic Acid Test Kit

Npaj siv:

SARS-CoV-2 Nucleic Acid Test kit yog siv rau qhov kev kuaj pom zoo ntawm nucleic acid ntawm COVID-19 hauv nasopharyngeal swabs, oropharyngeal swabs thiab hnoos qeev. Cov txiaj ntsig kev sim muab kev kuaj mob molecular rau cov kab mob lossis cov neeg mob xav tias yuav tsum tsis txhob siv los ua ib qho kev ntsuas rau kev kuaj mob.

Khoom siv:

Cov txheej txheem xeem:

A. Kev Npaj Ua Qauv

Extracted RNA yog cov khoom pib rau SARS-CoV-2 Nucleic Acid Test kit.

Tseem ceeb: Txhua txoj kev tshem tawm cov nucleic acid yuav tsum tau ua ib txhij nrog ib qho kev tswj zoo (40 μL, dilute nrog sterile saline daws / PBS rau qhov xav tau ntim) thiab ib qho kev tswj tsis zoo (40 μL, dilute nrog sterile saline daws / PBS rau qhov xav tau ntim).

Nws raug pom zoo kom siv cov khoom siv coj mus muag nucleic acid hauv qab no: PureLinkTM Viral RNA / DNA Mini Kit (Invitrogen); Viral RNA Mini cov khoom siv (QIAGEN); TIANamp Hi-DNA/RNA cov khoom siv (TIANGEN);

B. Reaction Mix teeb

1. Tag nrho cov reagents thiab cov qauv yuav tsum tau thawed kiag li, tov tag nrho thiab centrifuged luv luv ntawm chav tsev kub (kwv yees li ntawm 18 mus rau 25 degree) ua ntej siv.

2. Npaj cov ntim uas yuav tsum tau muaj ntawm Reaction Mix nyob rau hauv ib tug loj npaum li cas ntawm polypropylene microcentrifuge raj.

3. Pipette 25 µL ntawm Reaction Mix rau hauv txhua qhov xav tau ntawm ib qho chaw kho qhov muag uas tsim nyog 96-zoo tshuaj tiv thaiv phaj lossis lub raj tshuaj kho qhov muag uas tsim nyog.

C. Template ntxiv

1. Ntxiv 5 μL ntawm rho tawm RNA los ntawm Cov Qauv / Kev Tswj Zoo / Kev Tswj Tsis Zoo. Tag nrho cov ntim yog 30 μL.

2. Ua tib zoo sib xyaw cov qauv thiab kev tswj hwm nrog Kev Sib Tw Sib Nqus los ntawm pipetting nce thiab nqis / Vortex.

3. Kaw lub 96-cov tshuaj tiv thaiv zoo nrog lub hau tsim nyog lossis cov yeeb yaj kiab kho qhov muag thiab cov raj tshuaj tiv thaiv nrog lub hau tsim nyog.

4. Centrifuge lub 96-zoo tshuaj tiv thaiv phaj nyob rau hauv ib tug centrifuge nrog ib tug microtiter phaj rotor rau 30 vib nas this ntawm kwv yees li 2500 rpm.

D. Programming lub Real-Time PCR Instrument

Rau cov ntaub ntawv yooj yim hais txog kev teeb tsa thiab kev ua haujlwm ntawm cov cuab yeej PCR sib txawv ntawm lub sijhawm tiag tiag, thov xa mus rau cov neeg siv phau ntawv ntawm cov ntsuas ntsuas.Qhov kev ntsuam xyuas tau ua kom zoo rau ABI7500 Real-Time PCR system. PCR khiav yog ua nrog rau kev caij tsheb kauj vab raws li tau piav qhia hauv qab no.

Txhais cov fluorescence detectors

*Thov teeb lub fluorescence sab hauv siv ntawm lub ntsuas rau "Tsis muaj", piv txwv li: Rau ABI series twj paj nruag teeb "Passive Reference" rau "Tsis muaj".

E. Cov ntaub ntawv tsom xam

Thov teeb tsa cov kev tshuaj ntsuam xyuas thiab txheeb xyuas cov ntaub ntawv raws li cov lus qhia ua haujlwm ntawm cov cuab yeej cuam tshuam.

Siv ABI 7500 ua piv txwv:

Lub hauv paus yuav tsum tau teem rau ib tug ntau yam uas tshem tawm cov keeb kwm yav dhau fluorescence pom nyob rau hauv thaum ntxov cycles ntawm amplification, tab sis uas tsis overlap lub cheeb tsam uas amplifications signals sawv saum toj no lub keeb kwm yav dhau (Pib tus nqi: 3 ~ 10; kawg tus nqi: {{2} }). Lub Cycle Threshold (Ct) yuav tsum nyob hauv lub hauv paus ntawm theem exponential. Nyem "Tshawb xyuas" kom tau txais cov txiaj ntsig kev tshuaj ntsuam tau txais, thiab nyeem cov txiaj ntsig tshawb pom hauv "Tshaj Tawm" qhov rai.

Cim npe nrov: sars - cov-2 nucleic kua qaub xeem kab, Suav teb, neeg muab khoom, manufacturers, Hoobkas, loj